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Light-sheet microscopy

Light-sheet microscopy

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Muscle oscillations & waves

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Home posts: Science

Minimic side view

<img decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2019/06/minimic-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Minimic side view" data-attachment-id="1930" data-permalink="https://sguenther.eu/minimic/minimic-sideview/" data-orig-file="https://sguenther.eu/wp-content/uploads/2019/06/minimic.jpg" data-orig-size="1250,1250" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Minimic" data-image-description="<p>Side view of the <em>minimic</em>. An <a href="https://www.intuity.de/minimic-intelligenter-laborhelfer-fuer-routinemikroskopie/" target="_blank" rel="noopener noreferrer">Intuity</a> project for a better world: <a href="https://www.minimic.com" target="_blank" rel="noopener noreferrer">minimic.com</a>.</p> " data-image-caption="<p>Minimic side view</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2019/06/minimic-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2019/06/minimic-1024x1024.jpg" />

Blood film with malaria infected red blood cells

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2020/06/Malaria_blood_film-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Blood film with malaria infected red blood cells" data-attachment-id="2086" data-permalink="https://sguenther.eu/healthcare/malaria/blood_film_spot/" data-orig-file="https://sguenther.eu/wp-content/uploads/2020/06/Malaria_blood_film.jpg" data-orig-size="1500,1500" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Malaria blood film" data-image-description="<p>Content Providers: CDC/Dr. Mae Melvin – This media comes from the Centers for Disease Control and Prevention’s Public Health Image Library (PHIL), with identification number #2715. https://commons.wikimedia.org/w/index.php?curid=745649</p> " data-image-caption="<p>Blood film with an infected red blood cell. </p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2020/06/Malaria_blood_film-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2020/06/Malaria_blood_film-1024x1024.jpg" />

Heidelberg exhibition poster

<img loading="lazy" decoding="async" width="102" height="150" src="https://sguenther.eu/wp-content/uploads/2016/09/lifeinperspective_heidelberg_exhibition_poster_2-102x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Heidelberg exhibition poster" data-attachment-id="1731" data-permalink="https://sguenther.eu/data/life-in-perspective/heidelberg_exhibition_poster_2/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/09/lifeinperspective_heidelberg_exhibition_poster_2.jpg" data-orig-size="1784,2635" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Heidelberg exhibition" data-image-description="<p>We perceive our world as a three-dimensional reality. A truism forms the basis of “Life in Perspective”. The exhibition places the beauty of nature in the center using 3D posters about micro-organisms, thus translating the micro world into a familiar imagery. It is a change in perspective, which visualizes science in a comprehensive way by means of photography and computer design approaching an artistic dimension.</p> " data-image-caption="<p>Heidelberg exhibition of “Life in Perspective”</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/09/lifeinperspective_heidelberg_exhibition_poster_2-203x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/09/lifeinperspective_heidelberg_exhibition_poster_2-693x1024.jpg" />

Life in perspective - cover

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/06/lifeinperspective_cover-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Life in perspective - cover" data-attachment-id="1086" data-permalink="https://sguenther.eu/data/life-in-perspective/lifeinperspective_cover/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/06/lifeinperspective_cover.jpg" data-orig-size="2100,2100" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Life in perspective – cover" data-image-description="<p>Zebrafish embryo showing cell membranes in yellow and nuclei in magenta. Image was recorded by Dimitri Kromm at EMBL Heidelberg.</p> " data-image-caption="<p>Zebrafish embryo</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/06/lifeinperspective_cover-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/06/lifeinperspective_cover-1024x1024.jpg" />

Life in Perspective

Embryonic imaging

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/04/mousescope-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Embryonic imaging" data-attachment-id="346" data-permalink="https://sguenther.eu/embryonic-imaging/mousescope-2/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/mousescope.jpg" data-orig-size="2048,2048" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Embryonic imaging" data-image-description="<p>The image shows the cell’s nuclei from four time points of mouse embryonic development. In time the embryo grows and the number of cells increases. In the last time point also the cell membranes are shown.</p> " data-image-caption="<p>Mouse embryonic development</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/mousescope-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/mousescope-1024x1024.jpg" />

Embryonic imaging

Widefield vs confocal

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/04/confocal-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Widefield vs confocal" data-attachment-id="344" data-permalink="https://sguenther.eu/science/light-sheet-microscopy/confocal-2/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/confocal.jpg" data-orig-size="1900,1900" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Widefield vs confocal" data-image-description="" data-image-caption="<p>Image quality differences for widefield (left) and confocal (middle) recordings with a light-sheet microscope. Right shows the difference.</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/confocal-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/confocal-1024x1024.jpg" />

Quality images rock

Lineage specification

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/04/venustrap-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Lineage specification" data-attachment-id="345" data-permalink="https://sguenther.eu/lineage-specification/venustrap-2/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/venustrap.jpg" data-orig-size="1024,1024" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Lineage specification" data-image-description="<p>Embryonic and extra-embryonic cell lineages in mouse blastocyst. Image was recorded and visualized by Laura Panavaite at EMBL and used as a cover in <a href="http://embor.embopress.org/content/16/8.cover-expansion" target="_blank">EMBO reports 16 (8)</a>.</p> " data-image-caption="<p>Mouse embryo</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/venustrap-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/venustrap.jpg" />

Lineage specification

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/04/schnurri-150x150.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Dorsal closure" data-attachment-id="347" data-permalink="https://sguenther.eu/epithelial-tissue/schnurri-2/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/schnurri.jpg" data-orig-size="2510,2510" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Dorsal closure" data-image-description="" data-image-caption="<p>Fruit fly embryogenesis in toto</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/schnurri-300x300.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/schnurri-1024x1024.jpg" />

Epithelial tissue

Multi-view microscopy

<img loading="lazy" decoding="async" width="150" height="150" src="https://sguenther.eu/wp-content/uploads/2016/04/muvispim-150x150.png" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Multi-view microscopy" data-attachment-id="218" data-permalink="https://sguenther.eu/science/light-sheet-microscopy/muvispim/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/muvispim.png" data-orig-size="1900,1900" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Multi-view microscopy" data-image-description="<p>For multi-view imaging optical transparent access to the <em>Drosophila melanogaster</em> embryo is guaranteed through a gel that is nearly transparent in water.</p> " data-image-caption="<p>Fruit fly embryo embedded in a gel</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/muvispim-300x300.png" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/muvispim-1024x1024.png" />

Multi-view microscopy

<img loading="lazy" decoding="async" width="150" height="110" src="https://sguenther.eu/wp-content/uploads/2020/06/sarcomere_canvas-150x110.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Sarcomere" data-attachment-id="2106" data-permalink="https://sguenther.eu/science/muscle-waves/sarcomere_canvas/" data-orig-file="https://sguenther.eu/wp-content/uploads/2020/06/sarcomere_canvas.jpg" data-orig-size="2048,1500" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Sarcomere" data-image-description="<p>Schematics of striated muscle sarcomeres. Bundles of myosin motors are shown in blue, while actin filaments are red. In activated muscles bound myosin motors walk to the plus ends of the actin filaments shortening space between the Z-discs, which represents a sarcomere.</p> " data-image-caption="<p>Schematic muscle sarcomere</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2020/06/sarcomere_canvas-300x220.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2020/06/sarcomere_canvas-1024x750.jpg" />

Sarcomere dynamics

<img loading="lazy" decoding="async" width="150" height="110" src="https://sguenther.eu/wp-content/uploads/2016/04/swimmer-150x110.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Micro-swimmer" data-attachment-id="332" data-permalink="https://sguenther.eu/science/micro-swimmer/swimmer/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/swimmer.jpg" data-orig-size="1755,1284" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Micro-swimmer" data-image-description="" data-image-caption="<p>Possible shape changes of the three-sphere swimmer depending on the spheres’ distances left and right. For symmetry reasons the swimmer does not propagate for the motions depicted in the left and middle graph. Only the motion in the right graph allows for directed swimming.</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/swimmer-300x219.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/swimmer-1024x749.jpg" />

Spontaneous waves

<img loading="lazy" decoding="async" width="150" height="140" src="https://sguenther.eu/wp-content/uploads/2016/04/spontwaves-150x140.jpg" class="attachment-thumbnail size-thumbnail wp-post-image" alt="Spontaneous waves" data-attachment-id="339" data-permalink="https://sguenther.eu/science/muscle-waves/spontwaves/" data-orig-file="https://sguenther.eu/wp-content/uploads/2016/04/spontwaves.jpg" data-orig-size="2100,1953" data-comments-opened="0" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="Spontaneous waves" data-image-description="" data-image-caption="<p>Muscle waves</p> " data-medium-file="https://sguenther.eu/wp-content/uploads/2016/04/spontwaves-300x279.jpg" data-large-file="https://sguenther.eu/wp-content/uploads/2016/04/spontwaves-1024x952.jpg" />